Abstract

Research Article

Impact of amitriptyline on learning and memory

Mfem CC and Seriki SA*

Published: 14 April, 2021 | Volume 5 - Issue 1 | Pages: 009-015

Background/aim: Amitriptyline belongs to class of known as tricycline antidepresant (TCA) that is being used to treat anxiety and depressive states. It may help improve mood and feelings of well-being, relieve anxiety and tension, help to improve sleep and increase energy level. The study investigated the effect of amitriptyline on learning and memory using eighteen (18) healthy Swiss mice of both sexes weighing 16 – 25 g.

Method: The animals were divided into three (3) groups consisting of six (6) animals each. Group 1 served as the control group, Group 2 was administered with amitriptyline at a dose of 3 mg/kg body weight dissolved in 3 mls of distilled water, and used to test for learning, while Group three was also given similar administration like Group 2, but used to test for memory. All the animals were tested for learning and memory performance using Novel object recognition task and Morris water maze test.

Results: The results obtained from the Novel object recognition task showed that there was a significant decrease (p < 0.05) in total object approach in acquisition trial of amitriptyline treated group when compared to the acquisition trial of the control group. There was a significant decrease (p < 0.05) in retention trial of amitriptyline group when compared to retention trial in the control group. There was a significant decrease (p < 0.05) in total duration exploring objects in acquisition trial of amitriptyline treated group when compared to the acquisition trial of the control group. There was a significant increase (p < 0.05) in total duration exploring objects in retention trial of amitriptyline treated group when compared to the retention trial of the control group. There was a significant decrease (p < 0.05) in the index of habituation of amitriptyline treated group when compared to the control group. The index of discrimination showed a significant increase (p < 0.05) in amitriptyline treated group when compared to the control group and a significant decrease (p < 0.05) in amitriptyline group when compared to the control group. In the Morris water maze test, Day 1 – 3 were for acquisition training, day 4 – 6 reversal training, day 7 the probe trial day and day 8 the visible platform day. During acquisition training in the Morris water maze test, there was no significant difference in Swim latencies in day 1 and 2. However in day 3, there was a significant increase (p < 0.05) in swim latency of group compared to control group and a significant decrease (p < 0.05) in swim latency of amitriptyline treated group compared to the control group. During reversal training in day 1, 2 and 3, there was no significant difference in swim latency among the three groups. Results for the retention quadrant in the probe trials showed a significant decrease (p < 0.01) in amitriptyline group when compared to the control group.

Conclusion: Results suggest that amitriptyline impairs learning and memory functions.

Read Full Article HTML DOI: 10.29328/journal.ida.1001025 Cite this Article Read Full Article PDF

Keywords:

Amitriptyline; Learning and memory; Antidepressant; Sleep

References

  1. Atkinson RC, Shiffrin RM. Chapter: Human Memory: A proposed system and its control processes. In Spence, K. W.; Spenc J. T. The psychology of learning and motivation 2 New York Academic Press. 1968; 89-105.
  2. Eysenck A. Fundamentals of cognition. New York: Psychology Press 2007; 16-19
  3. Baddely A. Working memory, thought and action. Oxford, UK: Oxford University Press 2007; 30-35.
  4. Spear NE, Riccio DC. Memory: Phenomena and principles. Allyn and Bacconprintrs. 1994; 15-17.
  5. Montenegro M, Veiga H, Deslandes A. Nuromodulatory effects of caffeine and bromazepam on visual event-related potential (P300): a comparative study. Arq Neuropsiquiaatr. 2005; 63: 410-415. PubMed: https://pubmed.ncbi.nlm.nih.gov/16059590/
  6. Cunha M, Portela C, Bastos VH. Responsiveness of sensorimotor cortex during pharmacological intervention with bromazepam Neuroscience Letter 2008; 448: 33-36. PubMed: https://pubmed.ncbi.nlm.nih.gov/18938214/
  7. Zola M, Suire LR. Neuroantomy of memory. Ann Neurosci. 1993; 16: 547-563. PubMed: https://pubmed.ncbi.nlm.nih.gov/8460903/
  8. Kaplan SA, Jack ML, Weinfeld RE, Glover W, Weissman L, et al. Biopharmaceutical and clinical pharmacokinetic profile of bromazepam. J Phamacokinet Biopharm 2008; 4: 1-16. PubMed: https://pubmed.ncbi.nlm.nih.gov/5586/
  9. Graeff FG. Drogas psicotrópicas e seu modo de ação. 2.Ed. São Paulo: EPU, 1989.
  10. Katzung BG. Basic clinical pharmacology. 6.Ed. London: Pretence-Hall, 1995; 40-47.
  11. Oelschlager H. Chemical and pharmacologic aspects of benzodiazepines. Schweiz Rundsch Med Prax. 1989; 78: 766-772. PubMed: https://pubmed.ncbi.nlm.nih.gov/2570451/
  12. Ennaceur A. One-trial object recognition in rats and mice: methodological and theoretical issues. Behav Brain Ress. 2010; 215: 244-254. PubMed: https://pubmed.ncbi.nlm.nih.gov/20060020/
  13. Kopp C, Rudolph U, Low K, Tobler I. Modulation of rhythmic brain activity by diazepam: GABA (A) receptor subtype and state specificity. Proc Natl Acad Sci USA. 2004; 101: 3674-3679. PubMed: https://pubmed.ncbi.nlm.nih.gov/14990800/
  14. Hobi V, Dubach UC, Skreta M, Forgo I, Riggenbach H. The subacute effect of bromazepam on psychomotor activity and subjective mood. J Intern Med Res. 1982; 10: 140-146. PubMed: https://pubmed.ncbi.nlm.nih.gov/6124470/
  15. Jansen AAI, Verbaten MN, Slangen JL. Acute effects of bromazepam on signal detection performance digit symbol substitution test and smooth pursuit eye movement. Nueropsychobiol. 1988; 20: 91-95. PubMed: https://pubmed.ncbi.nlm.nih.gov/2908134/
  16. Heloisa VD, Andrea CD, Kaleb MC, Fernando AM. Neuromodulatory effects of caffeine and bromazepam on visual event related potential (P300): A comparative study. Arq Neuropsiquatar. 2005; 63: 410-415. PubMed: https://pubmed.ncbi.nlm.nih.gov/16059590/
  17. Bourin M, Auget JL, Colombel MC, Larousse C. Effects of single oral doses of bromazepam, buspirone and clobazam on performance tasks and memory. Pharmacopsych. 1989; 22: 141-145. PubMed: https://pubmed.ncbi.nlm.nih.gov/2577220/
  18. Koelega HS. Benzodiazepines and vigilance performance: a review. Psychopharmacology. 1989; 98: 145-156. PubMed: https://pubmed.ncbi.nlm.nih.gov/2569213/
  19. Van Leeuwen TH, Verbaten MN, Koelega HS, Kenemans JL, Slangen JL. Effects of bromazepam on single-trial event-related potentials in a visual vigilance task. Psychopharmacol 1992; 106: 555-564. PubMed: https://pubmed.ncbi.nlm.nih.gov/1579628/
  20. Polich J. P300 in clinical applications. In Niedermeyer E, Lopes da Silva F. (eds). Electroencephalography: basic principles, clinical applications and related fields. 4 Ed. Baltimore: Urban & Schwarzenberg. 1999: 1073-1091.
  21. Matthews A, Kirkby KC, Martin F. The effects of single-dose lorazepam on the memory and behavioural learning. J Psychopharmacol. 2002; 16: 345-354. PubMed: https://pubmed.ncbi.nlm.nih.gov/12503834/
  22. Hayakkawa T, Uchiyama M, Urata J. Effects of small dose of triazolam on P300. Psychiatry Clin Neurosci. 1999; 53: 185-187. PubMed: https://pubmed.ncbi.nlm.nih.gov/10459684/
  23. Richard EB, Lianne S, Heather M. Developing standardized behavioural tests for knockout and mutant mice. ILAR J. 2000; 41: 163-174. PubMed: https://pubmed.ncbi.nlm.nih.gov/11406708/
  24. Paylor R, Baskall-Baldini L, Yuva L, Wehner JM. Developmental differences in place-learning performance between C57BL/6 and DBA/2 mice parallel the ontogeny of hippocampal protein kinase C. Behavio Neurosci. 1996; 110: 1415-1425. PubMed: https://pubmed.ncbi.nlm.nih.gov/8986342/
  25. Authier N, Balayssac D, Sautereau M, Zangarelli A. Benzodiazepine dependence: focus on withdrawal syndrome. Ann Pharm Fr. 2009; 67: 408-413. PubMed: https://pubmed.ncbi.nlm.nih.gov/19900604/
  26. Liljequist R, Linnoila M, Mattila MJ, Saario I. Effect of two weeks’ treatment with thioridazine, chlorpromazine, sulphiride and bromazepam, alone or in combination with alcohol, on learning and memory in man. Psychopharmacologia. 1975; 44205-4208. PubMed: https://pubmed.ncbi.nlm.nih.gov/710/
  27. Umaharan T, Sivayokan S, Sivansuthan S. Amitriptyline Dependence and Its Associations: A Case Report and Literature. Review Case Rep Psychiatry. 2021; 2021: 6647952. PubMed: https://pubmed.ncbi.nlm.nih.gov/33564485/

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